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Perlecan Antibody (CSI 001-76-02) in ELISA
BMC biotechnology 2010 -
Figure 2 ELISA identifies only Pln.D1 but not Pln.D3-5 in enriched rhPln.198 . The enriched high M r rhPln.198 synthesized by HEK 293 cells was used to coat microtiter wells that were subsequently blocked then incubated with 1.7 mug/ml of different primary antibodies as depicted in the legend. ELISA was completed as described in Methods. Background signal for each antibody binding to uncoated wells was subt... View More
产品信息
CSI 001-76-02
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
A76
抗原
Lysed bovine corneal endothelial.
偶联物
形式
Liquid
浓度
1.01 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1086586
产品详细信息
CSI 001-76-02 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). CSI 001-76-02 cross-reacts with human thrombospondin. Other species have not been tested.
CSI 001-76-02 has been successfully used in ELISA, immunohistochemistry and Western blotting techniques. CSI 001-76-02 can be used in immunoprecipitation, ELISA and immunostaining of frozen PLP-fixed sections of bovine and human tissues. CSI 001-76-02 is more sensitive than CSI 001-71-02 or CSI 001-74-02 in ELISA.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
靶标信息
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); HSPG; Perlecan; perlecan proteoglycan; PLC; RP11-132G19.2
基因别名: HSPG; HSPG2; PLC; PRCAN; SJA; SJS; SJS1
UniProt ID: (Human) P98160
Entrez Gene ID: (Human) 3339, (Bovine) 444872
